Cell Culture and Scale-Up
Blue Sky Biotech has emerged over the past 7 years as the industry’s premier baculovirus-mediated (BEVS) protein expression CRO. Blue Sky’s premium baculoviral (insect cell recombinant protein expression) service boasts Infection Kinetics Monitoring® (IKM), a proprietary process technology that significantly improves turnaround time and enhances reproducibility in tandem with TIPS/BIIC technology.
Blue Sky is proud to offer our clients a diverse range of tissue culture and fermentation services. Please browse them using the menu on the left or read about them below.
Insect (BEVS) Expression
For information about our Insect Expression BioServices, please use the menu on the left.
Bacterial Expression
- Expression Optimization, 24–96 data points on SDS-PAGE/Western blot
- Scale-up of E. coli in 10 L and 55 L bioreactors
- Batch, fed-batch and continuous culture
- Metabolic Labeling (in vivo)
Mammalian Expression
- Optimization of expression in bioreactors
- Flask scale and Cell Stack adherent options
- Scale-up of suspension CHO/HEK cells in wave or stir tank bioreactors, 5–200 L
- Bulk Recombinant Antibody Expression and Hybridoma Scale Up
- Bulk Transient Transfection using Blue Sky’s TeraPrep™ Hi-Res bulk Plasmid DNA
- EVA™ Processes for Adaptation to Suspension & Media Adaptation (EVolution Accelerated)
- Intracellular and secreted products
- Stable pool, stable (clonal) cell line production, including kill curve
- Membrane preparation
- Functional testing/Viability/Reporter Assay
Yeast Expression
- Strain Creation and Optimization
- Scale-up of yeasts in 10 L bioreactors
- Intracellular and secreted products
These are just a few of the services we perform. For more information about services not listed on this page, please contact us to find out what Blue Sky can do for you.