Current cell-based assays can take up to 18 months. Utilizing our innovative TDA 2.0 platform, cycle time is reduced to a low as 1 month. This allows you to gain valuable time without sacrificing quality.
Engineered recombinant HIS-tagged proteins are produced such that the HIS-tag is on the correct terminus of the protein to reflect the polarity of the enzyme with respect to the membrane (for example, ecto-domains of a receptor would be C-terminally tagged while intracellular domains are N-terminally tagged).
TDA 2.0™ is a soluble stable liposome, which is made from derivatized lipids, which have Ni-NTA covalently, attached to the lipid head group. This allows the HIS-tagged proteins to bind to the liposome creating an environment much like a cellular membrane. Unlike a sepharose or agarose bead where HIS-tagged proteins must detach and reattach to translate across the surface, the lipids are fully fluid within the 2-dimensional surface of the liposome, so associated proteins can translate and rotate freely. The spatial and relational organization provided by the membrane surface, combined with this fluidity, promotes the formation of higher-order structures, such as homo- or hetero- dimers or multimers, and allows recruitment of accessory factors.
TDA 2.0 allows you identify crucial lead compound faster and more efficiently. There are many benefits associated with TDA 2.0 such as:
- Identification of Relevant Substrates
- Increased in Enzymatic Activity
Contact us to request more information on our TDA services.